A fully automated flow-based approach for accelerated peptide synthesis
Here we report a fully automated, flow-based approach to solid-phase polypeptide synthesis, with amide bond formation in 7 seconds and total synthesis times of 40 seconds per amino acid residue. Crude peptide purities and isolated yields were comparable to those for standard-batch solid-phase peptide synthesis. At full capacity, this approach can yield tens of thousands of individual 30-mer peptides per year.
Pentelute Lab, MIT, Cambridge, Chemistry, Molecular biology, technology development, peptide, protein-based therapeutics, chemical Biology, Bradley Pentelute
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A fully automated flow-based approach for accelerated peptide synthesis
A fully automated flow-based approach for accelerated peptide synthesis
Nature Chemical Biology (2017)
DOI:10.1038/nchembio.2318
Published: Received 03 September 2016 Accepted 05 December 2016 Published online 28 February 2017
Authors: Alexander J Mijalis, Dale A Thomas III, Mark D Simon, Andrea Adamo, Ryan Beaumont, Klavs F Jensen & Bradley L Pentelute
Abstract
Here we report a fully automated, flow-based approach to solid-phase polypeptide synthesis, with amide bond formation in 7 seconds and total synthesis times of 40 seconds per amino acid residue. Crude peptide purities and isolated yields were comparable to those for standard-batch solid-phase peptide synthesis. At full capacity, this approach can yield tens of thousands of individual 30-mer peptides per year.
Category
2017, Publications
